Human CD38^hiCD138⁺ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes

Figure 5

High frequency of CD39⁺ cells among the expanded B lymphocyte population. Viable cells were analyzed for CD31 and CD39 expression before their transfer into differentiation conditions (D0). (a) Profiles of total viable cells according to CD38 and CD138 (left) and to CD31 and CD39 (right) are shown. The CD138^+/− cells represented % of the cell population in the presence of CD154 interaction (b) and % in the presence of CD70 (c). They represented % of the population when being in the simultaneous presence of both interactions (d). These cells were further analyzed for their expression of CD31 and CD39 according to their expression of CD138 as indicated in (b, c, and d). These results are representative of 11 and 10 independent experiments for the stimulation with L4.5 cells (b) or 3H7 cells (c), respectively. The results in (d) are representative of three independent experiments.

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