Human CD38^hiCD138⁺ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes

Figure 4

In vitro generated plasma cells secrete high level of IgA and IgG. Immunoglobulin secretion in supernatants was measured by the Bio-Plex human isotyping kit. The cumulated Ig concentration was determined on (a) day 5 (D5) after the transition phase and (b) day 14 (D14) at the end of differentiation. (c) Relative secretion was determined on D14 by normalizing immunoglobulin concentrations with total cell count on day 5, corresponding to the plateau in proliferation. The results are presented as the mean ± S.E.M. of 6 independent samples. No differences were observed between CD70 and CD154 conditions.

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