Activation of Dendritic Cells by the Novel Toll-Like Receptor 3 Agonist RGC100

Figure 2

Assessment of the stability of RGC100 in FCS, mouse serum, or human serum. RGC100 (1.6 μM) was incubated in 80% serum at 37°C and samples were analyzed on 12% native PAGE at indicated time points (h, hours and d, days). The corresponding untreated dsRNA (0 h) is shown as a reference. (a) Incubation of RGC100 in FCS up to 5 h. (b) Incubation of RGC100 in mouse serum up to 5 h. (c) Incubation of RGC100 in human serum up to 5 h. (d) Incubation of RGC100 in FCS up to 7 days. (e) Incubation of RGC100 in mouse serum up to 7 days. (f) Incubation of RGC100 in human serum up to 7 days. (g) Incubation of dsRNA (25 bp) used as a control in FCS for 5 hours. (e) Incubation of dsRNA (25 bp) used as a control in mouse serum for 5 hours. (f) Incubation of dsRNA (25 bp) used as a control in human serum for 5 hours.