Research Article

Activation of Dendritic Cells by the Novel Toll-Like Receptor 3 Agonist RGC100

Figure 1

Determination of physicochemical properties of RGC100. (a) Analysis of RGC100 by 12% native PAGE. RGC100 displays a length of 100 bp as indicated. It consists of 100 rC bases paired to 100 rG bases, perfectly annealed in a double strand. As a reference, a 100 mer consisting of homopolymeric cytidine is shown. (b) Analysis of poly(I:C) by 1% native agarose gel electrophoresis. p(I:C) LMW: poly(I:C) with a low molecular weight. p(I:C) HMW: poly(I:C) with a high molecular weight. (c) Analysis of RGC100 by size-exclusion chromatography (SEC) with UV, RI and RALS detection. Data analysis provides information about molecular size and polydispersity (Mw/Mn = 1.015).
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