Clinical Study
DNA Methyltransferase 3B Gene Promoter and Interleukin-1 Receptor Antagonist Polymorphisms in Childhood Immune Thrombocytopenia
Table 1
Allele types, PCR conditions, and PCR primers designed to amplify fragments harboring the polymorphic sites.
| | DNMT3B promoter | IL-1 Ra |
| Type of polymorphism | Single-base C/T | 86-bp VNTR | Site of polymorphism | Position 149 | Intron 2 | PCR primers | | | Upstream | 5′-TGCTGTGACAGGCAGAGCAG-3′ | 5′-CTCAGCAACACTCCTAT-3′ | Downstream | 5′-GGTAGCCGGGAACTCCACGG-3′ | 5′-TCCTGGTCTGCAGGTAA-3′ | Digestion | AvrII | — | Allele size (bp) | C: 380 | I: 410 | II: 240 | T: | III: 325 | IV: 500 |
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