Molecular Analysis of Activation-Induced Cytidine Deaminase Gene in Immunoglobulin-E Deficient Patients

Table 1

AICDA primers, PCR conditions, and size of the fragments used in the study.

Exon	Primer designation	Primer sequence	Annealing		Product size (bp)
			Temperature (^∘C)	Position^*
1	hAID1F	5^′-GCCGTTGGGGTACCTGGTGG-3^′	55	−179	280
	hAID1R	5^′-ATGAGAGAAAGGGATAGCTA-3^′		+100
2	hAID2F	5^′-AGCCCAAGTAATGACTTCCTTA-3^′	55	+5661	321
	hAID2R	5^′-ACCATCAGCAGGTGGCTCTAA-3^′		+5981
3	hAID3F	5^′-GACTAAGGCTACCAGAGCCG-3^′	55	+7221	440
	hAID3R	5^′-GCCCACTTCTTCCCCTCGAG-3^′		+7660
4	hAID4F	5^′-GTGAATGGCTCAGAGACAAGG-3^′	55	+7716	364
	hAID4R	5^′-ATCAGATGAAAACTGAGAGTGA-3^′		+8079
5	hAID5F	5^′-GTTACAAAGCCATCCACTCAG-3^′	55	+8334	237
	hAID5R	5^′-GAGAAGACTTGAAGGACTG-3^′		+8570

^*Position of primers is according to GENBANK sequence Accession Number AB040430.1.