Journal of Food Quality

Research Article

Detection of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in Inactivated Fermented Milk Using Fluorescence Quantitative Loop-Mediated Isothermal Amplification

Table 2

Information on specific primers.
Target strainPrimer nameSequence (5′⟶3′)Primer sequence length (nt)Amplified region length (bp)
L. bulgaricus (GenBank Acc. No: LC685718)FIPTGG​AGA​TCA​AGG​TGT​CCG​CGA​ATC​CTG​TCT​CAG​CCA​AAC​AC41174
BIPCCA​TCG​ACA​TCG​TCG​TGG​TCG​TTC​ACC​TTC​GAT​TTC​GGC​C40
F3GCG​TGG​ACA​TCG​ACC​AAT​T19
B3TCCAACGTGGGAGTCACC18
BLTTT​GCA​GCC​CTT​CTT​CCC​CA20
S. thermophilus (GenBank Acc. No: AGFN01000211.1)FIPTCG​AAA​TTA​AGG​GTG​AAA​ATG​GTC​ACT​TCA​TCC​GAC​TTA​CTC​TCT​G46215
BIPACT​GAT​GAT​TGA​TAA​AGA​AGC​TCC​AGA​TTC​ACC​GTC​GTG​ATG​C43
F3AGC​TAA​CAA​TGA​GGG​CAT​C19
B3GTGTTGCTGAGAGTGTGA18
FLCAC​TAT​GCT​CAT​GGG​CAC​GAA​AG23