Detection of Aflatoxin and Ochratoxin A in Spices by High-Performance Liquid Chromatography

Table 1

Validation parameters for the HPLC determination of AFB1, AFB2, AFG1, AFG2, and OTA.

Validation parameters	AFB1	AFB2	AFG1	AFG2	Total AFs	OTA
LOD (μg/kg)	0.1	0.2	0.3	0.2	0.6	0.47
LOQ (μg/kg)	0.4	0.7	0.9	0.6	1.8	1.23
Correlation coefficient (R^2)	0.9989	0.9991	0.9953	0.9995	0.09978	0.9968
Recovery (%)	82.5–84.3	74.1–83.1	77.7–88.8	75.0–75.9	74.1–83.1	83.7–99.6
RSD (%, n = 20)	1.31	3.77	2.35	2.81	1.77	1.64
RME (%, n = 20)	−3.4	−1.0	1.8	−2.1	1.6	2.6

Note: RSD, relative standard deviation; RME, reversed micellar extraction; LOD, limit of detection; LOQ, limit of quantification.The bold values represent OTA.