Partial Purification and Characterization of the Mode of Action of Enterocin S37: A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces

Table 3

Purification of enterocin S37 produced by Enterococcus faecalis S37.

Purification step	Volume (ml)	Protein concentration (𝜇g⋅ml^-1)	Activity (AU^∗⋅ml^-1)	Specific activity (AU^∗⋅𝜇g^-1)	Purification factor
Filtered culture supernatant	400	10,647.94	400	0.03	1
Ammonium sulfate precipitation	40	5,079.03	12,800	2.52	84
First RP-HPLC	10	56.64	51,200	903.95	30,132
Second RP-HPLC	1	32.88	51,200	1,557.17	51,906

∗Arbitrary Unit was calculated as described by Batdorj et al. [12].