Research Article

Diabetes Mellitus and Increased Tuberculosis Susceptibility: The Role of Short-Chain Fatty Acids

Figure 4

Influence of butyrate on cellular metabolism. (a and b) Kinetics of lactate production (a) and intracellular NAD+/NADH ratios (b) from days 1, 3, and 7 of PBMCs preincubated with 50 μM C4 (1 h) with and without stimulation with Mtb lysate. Data are means ± SEM ( to 5), using Wilcoxon signed-rank test, representative of 1-2 independent experiments. (c) Expression levels of glycolysis and TCA cycle genes in PBMCs preincubated with 50 μM C4 (1 h) and stimulated with Mtb lysate or LPS (4 h) as measured by qPCR. Data are means ± SEM (), using Wilcoxon signed-rank test, representative of 2 independent experiments.
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