Large Gliadin Peptides Detected in the Pancreas of NOD and Healthy Mice following Oral Administration

Table 2

Fragments formed in vitro during incubation of 33-mer in mouse plasma, as determined by MALDI-TOF MS.

		Sample
1		Filtrate
2		Filtrate
3		Filtrate
4		Filtrate
5		Filtrate
6		4–6 kDa
7		Filtrate
8		14–18 kDa
9		10–12 kDa
10		10–12 kDa
11		14–18 kDa
12		14–18 kDa
13		14–17 kDa
14		14–17 kDa
15		4–6 kDa
16		4–6 kDa, 6–9 kDa, 14–17 kDa

33-mer was incubated at 37°C in mouse plasma, and samples were filtered through 5 kDa MWCO centrifugal filters or run on SDS-PAGE gels before MALDI-TOF MS analysis. Approximate ranges of the gel slices, according to the molecular weight marker, are shown, and assignment of observed masses to 33-mer fragments was done as described in Table 1. The appearance of bands at 10–20 kDa on SDS-PAGE gels during incubation was confirmed before analysis of gel slices (not shown). The identified fragments at 10–18 kDa were not found in plasma where 33-mer was not added or when sampled at .