Large Gliadin Peptides Detected in the Pancreas of NOD and Healthy Mice following Oral Administration

Figure 4

Analysis of plasma after administration of 33-mer to BALB/c mice by LC-MS. (a) Total ion current chromatograms with the 33-mer eluting at approximately 22.9 min. (b) Averaged mass spectra corresponding to the elution profiles. Plasma was precipitated with methanol-TFA before analysis. Rows 1–4 show control samples: 33-mer at 150 µg/mL (rows 1 and 2), plasma (row 3), and plasma added 150 µg/mL 33-mer (row 4). Rows 5–7 show plasma obtained 15, 30, and 60 min, respectively, after i.v. administration of 850 µg 33-mer, and row 8 shows plasma obtained 60 min after p.o. administration of 650 µg 33-mer. The levels after p.o. administration were close to the detection limit, and the peptide was not detected in all mice.

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