Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice

Figure 8

Impaired sensory perceptions in diabetic mice were ameliorated by angioblast-like cells induced from ES cells (ES-ABs) transplantation. Stimuli with frequencies of 5 Hz (a), 250 Hz (b), and 2000 Hz (c) evoked excitations of C-fiber, Aδ-fiber, and Aβ-fiber, respectively. Before cell transplantation, CPTs with all kinds of stimuli in diabetic mice had significantly increased compared with those in normal mice, representing hypoalgesia in diabetic mice. Four weeks after the transplantation of ES-ABs, these deficits in sensation were significantly improved in diabetic mice compared with saline-treated diabetic controls. The transplantation of ES-ABs into normal mice did not induce significant changes in CPTs. N: normal mice limbs, DM: diabetic mice limbs, S: saline-injected limbs, AB+: limbs transplanted ES-ABs, and AB−: contralateral limbs transplanted ES-ABs. Results are means ± SD. versus pretreatment N, versus posttreatment S-treated N, and versus posttreatment S-treated DM. –10 in each nondiabetic group and –9 in each diabetic group.

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