Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice

Figure 5

The transplanted angioblast-like cells induced from ES cells (ES-ABs) constructed blood vessel walls and capillaries. Immunohistochemical staining revealed that transplanted GFP-expressing ES-ABs were still engrafted 4 weeks after transplantation. (a) The engrafted cells distributed in vessel wall between hindlimb muscle fibers and coexpressed α-SMA. Green: GFP and red: α-SMA. M: muscle and V: lumen of a blood vessel. Bar: 20 μm. (b) Some of GFP positive cells resided in gaps between soleus muscle fibers and coexpressed PECAM. Green: GFP and red: PECAM. White arrows: GFP negative capillaries and yellow arrowheads: GFP positive capillaries. Bar: 20 μm.

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