Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery

Figure 5

MAb 734 binding to DTPA-indium-tagged liposomes adsorbed onto a L1 chip. Kinetic titration series of MAb 734 on DSPC/DSPE-DTPA-indium-containing liposomes with various DSPE-PEG 2000 concentrations deposited on an L1 chip. The liposomes were deposited on the L1 Chip at the same level of Resonance Units, approximately 15 000 RU. For each formulation, MAb 734 was prepared in two-fold serial concentration series (720 nM to 2.185 nM) and was injected at a 60 μL/min flow rate across the liposomes adsorbed on the L1 chip surface for 3 min. At the end of each injection, the dissociation phase was set to 4 min.