Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery
Deposition and absorption of liposomes on an L1 chip. Liposomes were deposited on the L1 chip for 5 min at 5 μL/min in PBS. The concentration of lipids was 1 mg/mL. The flow rate was changed to 30 μL/min after the deposition, and liposomes were washed with three consecutive injections of NaOH in increasing concentration (20, 50, and 100 mM). All vesicles were in PBS during the injection. A control surface was prepared by loading PEGylated liposomes without DTPA-indium on the first flow cell of a L1 sensor chip (DSPC/Chol/DSPE-PEG2000).
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