Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery
Tethered planar bilayer formation on DMPE functionalized CM5 chip. A BIAcore CM5 chip was coated with DMPE as described in materials and methods. The functionalized chip was treated with DSPC/DSPE-DTPA containing vesicles at a 5 μL/min flow rate. The sensorgram shows the spreading of liposomes to a level of 1100 RU and the formation of a stable planar bilayer.
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