Confirmed Mechanism for 1,8-Diaminonaphthalene and Ethyl Aroylpyrovate Derivatives Reaction, DFT/B3LYP, and Antimicrobial Activity of the Products

Table 4

The activity of the tested compounds 12a–e and 21a–e against four G⁺ bacteria and four G^- bacteria fungi species.

Compound number	Gram-positive bacteria					Gram-negative bacteria
	Staphylococcus aureus	Staphylococcus epidermidis	Bacillus subtilis	Streptococcus pyogenes	Pseudomonas aeruginosa	Escherichia coli	Klebsiella pneumoniae	Salmonella typhimurium
12a	19.3 ± 0.58 (81.4%)	17.4 ± 0.58 (77.7%)	26.7 ± 1.2 (82.4%)	NA	NA	24.3 ± 0.63 (95.7%)	21.9 ± 0.58 (96.9%)	20.4 ± 0.72 (87.6%)
12b	22.8 ± 0.58 (96.2%)	21.6 ± 0.72 (96.4)	29.3 ± 0.58 (90.4%)	NA	NA	24.2 ± 0.72 (95.3%)	20.3 ± 0.63 (89.8%)	21.6 ± 0.63 (92.7%)
12c	22.6 ± 0.85 (95.4%)	19.6 ± 1.2 (87.5%)	28.3 ± 0.63 (87.3%)	NA	NA	25.2 ± 0.58 (99.2%)	18.6 ± 1.2 (82.3%)	19.9 ± 0.63 (85.4%)
12d	21.2 ± 0.63 (89.5%)	19.6 ± 0.58 (87.5%)	27.3 ± 0.53 (84.3%)	NA	NA	24.2 ± 1.2 (95.3%)	22.1 ± 0.44 (97.8%)	19.9 ± 0.72 (85.4%)
12e	23.1 ± 0.63 (97.5%)	22.7 ± 0.72 (101.3%)	28.1 ± 0.53 (86.7%)	NA	NA	18.5 ± 1.2 (72.8%)	19.2 ± 1.2 (85.0%)	20.4 ± 0.58 (87.6%)
21a	16.7 ± 0.63 (70.5%)	14.4 ± 0.72 (64.3%)	19.2 ± 0.63 (59.3%)	NA	NA	17.8 ± 1.2 (70.1%)	17.3 ± 1.2 (76.5%)	19.6 ± 0.58 (84.1%)
21b	15.2 ± 1.2 (64.1%)	19.8 ± 0.67 (88.4%)	24.3 ± 0.58 (75.0%)	NA	NA	17.9 ± 0.58 (70.5%)	13.3 ± 0.58 (58.8%)	18.4 ± 1.2 (79.0%)
21c	22.4 ± 0.63 (94.5%)	20.9 ± 0.72 (93.3%)	23.8 ± 0.63 (73.5%)	NA	NA	24.3 ± 1.2 (95.7%)	22.5 ± 1.2 (99.6%)	26.3 ± 0.58 (112.9%)
21d	13.5 ± 1.2 (57.0%)	15.3 ± 0.44 (68.3%)	18.2 ± 0.58 (56.2%)	NA	NA	19.3 ± 0.63 (76.0%)	16.3 ± 0.63 (72.1%)	19.3 ± 0.58 (82.8)
21e	15.3 ± 0.43 (64.6%)	18.6 ± 0.58 (83.0%)	22.4 ± 0.53 (69.1%)	NA	NA	16.4 ± 0.53 (64.6%)	20.4 ± 0.53 (90.3%)	18.7 ± 0.63 (80.3%)
Ampicillin	23.7 ± 0.63	22.4 ± 1.2	32.4 ± 0.72	24.5 ± 0.63	—	—	—	—
Gentamicin	—	—	—	—	22.3 ± 0.58	25.4 ± 1.2	22.6 ± 0.63	23.3 ± 0.58

NA: no activity; data are expressed in the form of mean ± SD.