Chemical Profiling of Polyfloral Belgian Honey: Ellagic Acid and Pinocembrin as Antioxidants and Chemical Markers

Table 2

Calibration curves and detection limits of studied flavonoids and phenolic acids for HPLC-PAD analysis.

Analyte	Retention time; min	Correlation coefficient	LOD; ug/ml	LOQ; ug/ml	Precision (RSD)%
					Interday	Intraday
3,4-Dihydroxybenzoic acid	10.56	0.9993	0.03	0.07	1.9	3.4
Chlorogenic acid	14.36	0.9992	0.16	0.47	2.6	3.2
4-Hydroxybenzoic acid	15.11	0.9912	0.02	0.07	2.1	2.9
3-Hydroxybenzoic acid	15.65	0.9998	0.04	0.13	2.5	3.1
Vanillic acid	16.06	0.9983	0.10	0.28	1.9	2.8
Caffeic acid	16.34	0.9992	0.13	0.41	2.5	3.2
Syringic acid	17.06	0.9994	0.11	0.35	2.1	2.9
Ferulic acid	19.44	0.9993	0.16	0.48	1.6	2.4
p-Coumaric acid;	19.85	0.9968	0.08	0.25	2.2	2.7
Rosmarinic acid	21.21	0.9987	0.14	0.43	2.4	3.1
Ellagic acid,	24.05	0.9979	0.15	0.47	2.5	3.1
Myricetin	25.46	0.9991	0.38	1.13	1.8	2.8
(+)-naringenin	26.70	0.9982	0.11	0.34	2.2	3.2
Quercetin	29.20	0.9967	0.26	0.80	1.6	2.4
Kaempferol	32.05	0.9998	0.31	0.92	1.9	2.6
Pinocembrin	32.75	0.9996	0.08	0.23	1.6	2.5
Chrysin	35.57	0.9985	0.28	0.61	2.1	2.9
Galangin	36.10	0.9990	0.15	0.48	2.3	3.1

LOD, limit of detection at the signal three times at the baseline noise; LOQ, limit of quantification.