Flexural Stiffness of Myosin Va Subdomains as Measured from Tethered Particle Motion

Figure 6

MyoVa construct tethered particle motion relative to actin baseline () (open circles, ○) increases with increasing tether length. Solid gray line indicates a theoretical maximum of 1 : 1, where the tethered particle motion equals that of the tether length itself. MyoVa-S1 tethered particle motion in the presence of calcium (square, □). The data were fit with a slope of 0.341 nm/nm, with the 95% confidence interval for the fit shaded in gray.