Flexural Stiffness of Myosin Va Subdomains as Measured from Tethered Particle Motion

Figure 3

Image size parameter versus effective camera exposure time. The number of frames necessary to sample the full extent of Qdot motion was established by summing increasing numbers of 8.3 ms exposures of 33 Qdots tethered by full-length MyoVa. Lower effective exposure times tended to underestimate the image size parameter (shown as median ± standard error of the median as calculated by (1) in text). The effective exposure time used for analysis (2.49 s) was approximately ten times longer than that required to reach a point where the image size parameter was no longer changing. Inserts show the same Qdot imaged for one frame (left) and for the full 2.49 s integration (right).