Flexural Stiffness of Myosin Va Subdomains as Measured from Tethered Particle Motion

Figure 1

Tethered Qdot motion. Brownian motion causes a Qdot (red hexagon) fixed to an elastic tether to move (a), resulting in an increase in apparent point spread function (b). In both the tethered and surface bound states, the point spread functions are Gaussian in shape (profile along dashed line shown in (b), with standard deviations corresponding to image size parameters and , respectively.

(a)

(b)

(c)