Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells
Figure 9
Inactivation in the steady state of current in the cellular line of rat GC somatotropes. (a) Family of representative traces of the inactivation in the steady state of current. The current was evoked by a double-pulse protocol; the first of these, named the conditioning pulse, starts from −130 mV and has a duration of 1.5 seconds and a varied amplitude (10 mV); whereas the second one, named testing, carries the membrane potential at 70 mV and lasts 500 ms. The holding potential was fixed at −80 mV. (b) Adjustment of the current at the peak of the testing pulse in accordance with the conditioning pulse voltage and normalization of the latter as regards to the maximum current; the continuous lines represent the adjustment of the data with a Boltzmann-type function. (c) Maximum current evoked by the testing pulse in experimental conditions; the numbers beside the error bar represent the number of cells analyzed. (d) Boltzmann-type adjustment of values: midpoint and slope factor ().
