Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels
Figure 6
GSNO treatments extend chronological lifespan (CLS). (a and b) Treating cells with low doses of GSNO extends CLS. (a) Cells are treated with a single dose of GSNO at a final concentration of 25 μM upon starting the CLS assay (at 0 hr). (b) Cells are treated with GSNO at a final concentration of 5 μM at six different time points (6x): 0, 3, 6, 9, 18, and 24 hr. (c) GSNO treatment does not further increase the long CLS of representative CR mimic mutants. (d) GSNO treatments partially rescue the short CLS of the cyt1Δ mutant. Results show relative viability of cells taken from GSNO-treated CLS culture (in 5-fold serial dilutions). T0: treating cells with GSNO upon starting CLS; T24: treating cells with GSNO 24 hr after starting CLS. (e) Cells pretreated with 25 μM GSNO (+GSNO) show resistance towards GSNO-induced toxicity. (f) Cells pretreated with 25 μM GSNO (+GSNO) show mild resistance towards hydrogen peroxide-induced toxicity. WT: BY4742 wild-type control; CR: 0.5% glucose. One representative set of three independent experiments, each conducted in quadruplicate (for a, b, and c), is shown. Error bars denote standard deviations. For (a) to (c), numbers on -axes denote the number of days after entering stationary phase (nonmitotic state).
