Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels
Figure 1
Calorie restriction (CR) and CR genetic mimics increase nitric oxide (NO) levels and confer resistance to nitrosative stress in yeast cell. (a) CR increases NO-related signals during early growth stage. (b) Long-lived tor1Δ and hxk2Δ mutants also show increased NO-related signals. (c) CR and CR mimics confer resistance to S-nitrosoglutathione- (GSNO-) induced toxicity. Results show percentages (%) of growth normalized to no treatment controls after treated with GSNO at indicated concentrations for 20 hr. (d) Yeast cells grown in CR show increased NO metabolizing activities determined in cell lysates. values are calculated comparing CR cells and the tor1Δ or hxk2Δ mutants to WT control. WT: BY4742 wild-type control; CR: 0.5% glucose; Δ: gene deletion. One representative set of three independent experiments is shown. Error bars denote standard deviations.
