Research Article

Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells

Table 1

Antibodies: source and use.

Primary antibodiesSecondary antibodies
(dilution, if not stated otherwise, 1 : 400)

Mouse monoclonal anti-hNANOG (N3038, Sigma) 1 : 75Goat anti-mouse IgG-Alexa Fluor 488 (A31619, Invitrogen) or 594 (A31623, Invitrogen)
Rabbit polyclonal anti-hOCT4 (ab19857, Abcam) 1 : 200Goat anti-rabbit-IgG-Alexa Fluor 594 (A31631, Invitrogen)
Goat polyclonal anti-hOCT3/4 (N-20) (sc-8630X, Santa Cruz) 1 : 400Rabbit anti-goat IgG-Cy3 (C2821, Sigma) 1 : 500
Rabbit polyclonal anti-hP21 C-19 (sc-397, Santa Cruz) IF 1 : 50Goat anti-rabbit-IgG-  .Alexa Fluor 594 (A31631, Invitrogen)
Rabbit polyclonal anti-hP16 N-20 (sc-467, Santa Cruz) IF 1 : 50Goat anti-rabbit-IgG-  .Alexa Fluor 594 (A31631, Invitrogen)
Mouse monoclonal anti-hPML (sc-966, Santa Cruz) 1 : 150Goat anti-mouseIgG-Alexa Fluor 488 (A31627, Invitrogen)
Rabbit polyclonal anti-hAURORA B (ab2254, Abcam) 1 : 300Goat anti-rabbit-IgG-  .Alexa Fluor 594 (A31631, Invitrogen)
Rabbit polyclonal anti-h-γH2AX (4411-PC-100, R&D Systems) 1 : 200Goat anti-rabbit-IgG-  .Alexa Fluor 594 (A31631, Invitrogen)