Optimization of the Imaged cIEF Method for Monitoring the Charge Heterogeneity of Antibody-Maytansine Conjugate

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left">Ampholyte pH range</td><td class="align_center">Pharmalytes 8–10.5/pharmalytes 3–10</td></tr><tr><td class="align_left">Sample concentration</td><td class="align_center">0.3, 0.5, 0.8, 1, and 1.5 mg/ml</td></tr><tr><td class="align_left">Urea concentration</td><td class="align_center">0, 1, 2, and 3 M</td></tr><tr><td class="align_left">Focusing time</td><td class="align_center">7, 10, 12, and 15 min</td></tr><tr><td class="align_left">% ampholytes</td><td class="align_center">4%, 6%, and 8%</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Optimized experimental parameters for ADC analyses by iCE280.</div>

Journal of Analytical Methods in Chemistry

tab2

Table 2

Table 2: Optimization of the Imaged cIEF Method for Monitoring the Charge Heterogeneity of Antibody-Maytansine Conjugate 