Simultaneous Determination of Three Compounds in Rat Plasma by UHPLC-QQQ-MS/MS and Its Application to Pharmacokinetics of Banxia Baizhu Tianma Tang

Table 1

Precision, accuracy, recovery, and matrix effect of ephedrine, gastrodin, and liquiritin in rat plasma.

Analytes

Added C (ng/mL)

Extraction recovery (%) (n = 6)

Matrix effect (%) (n = 6)

Intraday (n = 5)

Interday (n = 15)

Stability (n = 6)

Found C (ng/mL)

RSD (%)

RE (%)

Found C (ng/mL)

RSD (%)

RE (%)

Short-term stability (%)

Long-term stability (%)

Freeze-thawstability (%)

Ephedrine

5

86.88 ± 1.08

100.4 ± 3.83

4.57 ± 0.52

11.5

−8.60

4.48 ± 0.60

13.4

−10.4

89.23 ± 2.63

87.43 ± 5.26

87.15 ± 3.81

40

87.47 ± 2.35

96.40 ± 2.45

41.3 ± 2.8

6.84

3.33

37.6 ± 3.3

8.89

-6.0

91.40 ± 3.05

90.68 ± 2.43

90.04 ± 4.07

200

92.32 ± 3.69

96.76 ± 3.82

209.8 ± 10.3

4.93

4.90

205.6 ± 8.81

4.28

2.82

91.27 ± 5.22

91.67 ± 1.07

90.66 ± 6.42

Gastrodin

150

89.25 ± 4.07

96.59 ± 1.26

162.7 ± 10.2

6.28

8.4

161.3 ± 18.0

11.1

−7.56

89.29 ± 3.99

90.11 ± 3.78

89.88 ± 2.33

600

87.98 ± 5.20

96.72 ± 3.57

580.3 ± 20.6

5.15

−3.3

587 ± 35.8

6.09

−2.12

90.69 ± 5.33

90.85 ± 1.04

91.58 ± 1.27

2000

90.14 ± 3.22

95.15 ± 0.78

1989 ± 66.6

3.35

−0.53

2016 ± 87.2

4.32

−0.80

91.80 ± 4.01

93.11 ± 3.78

93.33 ± 2.63

Liquiritin

15

88.75 ± 6.08

98.17 ± 3.69

16.1 ± 1.7

10.7

7.56

16.4 ± 1.5

9.33

9.11

89.16 ± 1.28

89.23 ± 4.65

88.29 ± 5.96

75

90.49 ± 3.83

97.71 ± 1.54

70.7 ± 3.3

4.74

−5.78

76.4 ± 1.9

2.46

1.82

91.72 ± 6.47

91.87 ± 1.93

90.59 ± 4.22

400

90.81 ± 1.73

99.87 ± 7.01

390.8 ± 15.5

3.97

−2.3

389.5 ± 15.0

3.86

−2.63

92.13 ± 1.91

93.33 ± 5.74

92.70 ± 2.91