Chemometric Methods for Simultaneous Determination of Candesartan Cilexetil and Hydrochlorothiazide in Binary Combinations

Table 2

Limit of detection (LOD) and limit of quantification (LOQ) of HCT by HPSAM.

Λ	Regression equation	R^2	Added (µg/mL)		Found (µg/mL)
			HCT	CAN	HCT	CAN
283	Y = 0.01158X + 0.1783	0.9915	15	15	0.21	14.9
239	Y = 0.03315X + 0.4996	0.9955
283	Y = 0.01152X + 0.1767	0.9905	15	15	0.2	14.85
239	Y = 0.03315X + 0.4978	0.9955
283	Y = 0.01147X + 0.1813	0.9911	15	15	0.33	15.02
239	Y = 0.03295X + 0.5039	0.9962
283	Y = 0.01141X + 0.1787	0.9917	15	15	0.23	15.12
239	Y = 0.03276X + 0.5014	0.9968
283	Y = 0.01136X + 0.1800	0.9922	15	15	0.33	15.05
239	Y = 0.03282X + 0.5029	0.9967
Mean					0.26
SD					0.065
LOD					0.46
LOQ					0.91

¹ calculated using the regression equation of Y = 0.0247X + 0.0297 and the HCT calibration curve at 283 nm.