Journal of Analytical Methods in Chemistry

Research Article

A Validated 1H NMR Method for the Quantitation of 5-Hydroxymethyl-2(5H)-Furanone, a Valuable Chemical Intermediate, In a Dichloromethane Extract of Helleborus lividus subsp: Corsicus Leaves from Corsica

Table 4

Quantitation of 5-hydroxymethyl-2(5H)-furanone (F) in the dichloromethane leaf extract from H. lividus subsp. Corsicus by 1H NMR.
Signal at 6.16 ppm (H2)Signal at 7.50 ppm (H3)
AF2.84AF2.86
mF (mg)25.22mF (mg)25.39
F content (%)84.35F content (%)84.93
Mass of the extract: 29.9 mg; area of the signal of the aldehydic proton of vanillin is fixed at 1.00 in all experiments; amount of vanillin (mV) = 11.84 mg; purity of vanillin: 99%; molecular weight of 5-hydroxymethyl-2(5H)-furanone: 114.10 g·mol−1; molecular weight of vanillin: 152.15 g·mol−1; AF: areas of the selected signals at 6.16 ppm and 7.50 ppm (ethylenic protons); mF: calculated mass (mg) of F according to equation (1).