A Validated ¹H NMR Method for the Quantitation of 5-Hydroxymethyl-2(5H)-Furanone, a Valuable Chemical Intermediate, In a Dichloromethane Extract of Helleborus lividus subsp: Corsicus Leaves from Corsica

Table 2

Accuracy of 5-hydroxymethyl-2(5H)-furanone (F) measurements by ¹H NMR.

Signal at 6.16 ppm (H2)				Signal at 7.50 ppm (H3)
F area	Weighted mass (mg)	Calculated mass (mg)	RE (%)	F area	Weighted mass (mg)	Calculated mass (mg)	RE (%)
0.22	2.03	2.03	0.00	0.23	2.03	2.12	4.25
0.44	4.07	4.05	−0.49	0.44	4.07	4.05	−0.49
0.87	8.14	8.01	−1.62	0.88	8.14	8.01	−1.62
1.32	12.20	12.16	−0.33	1.32	12.20	12.16	−0.33
1.76	16.27	16.21	−0.37	1.76	16.27	16.21	−0.37
2.21	20.34	20.36	0.10	2.21	20.34	20.36	0.10
3.31	30.51	30.49	−0.07	3.31	30.51	30.49	−0.07

The area of the signal of the aldehydic proton of vanillin is fixed at 1.00 in all experiments; F area: area of the selected signals of 5-hydroxymethyl-2(5H)-furanone; mass of vanillin: 12.60 mg; calculated mass according to formula (1); RE: relative error.