Quantitative Analysis of Berberidis Cortex via Quantitative Analysis of Multicomponents by Single Marker (QAMS) Combined with Fingerprint and Chemometrics Methods

Table 5

The RCF values of three alkaloids in different conditions.

Chromatographic condition	Factors	Chromatographic columns	fs/A	fs/B	fs/C
Column	Waters 2695	CAPCELL PAK C18	2.4867	0.9971	1.0096
		Phenomenex luna C18	2.5126	0.9975	1.0179
		Venusil XBP C18	2.4742	0.9914	1.0077
	Waters ACQUITY	CAPCELL PAK C18	2.4459	0.9849	0.9938
		Phenomenex luna C18	2.4908	0.9825	1.0169
		Venusil XBP C18	2.4175	0.9762	0.9867
	Mean		2.4713	0.9883	1.0054
	RSD (%)		1.4	0.86	1.3
Column temperature	20°C		2.4394	0.9840	1.0026
	25°C		2.4489	0.9962	1.0028
	30°C		2.4447	0.9876	1.0037
	35°C		2.4315	0.9683	0.9978
	Mean		2.4411	0.9840	1.002
	RSD (%)		0.31	1.2	0.27