Research Article

Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study

Table 2

The six methods for calculating the relative correction factor.

MethodFormula of RCF

A [22]lgAk = lgCk−detected × Fs + b
RCF# = Ck−detected/Ck
B [23, 24]RCF = Fk/Fs
C [25]RCF# = (Cs/lgAs)/(Ck/lgAk)
D [3]RCF# = (lgCk/lgAk)/(lgCs/lgAs)
E [26]RCF# = (As/Cs)/(Ak/Ck)
F [22]RCF = 1.00

b was the intercept of the linear equation for the single marker. #The RCF of each analyte was the average of RCFs at different concentration levels.