Simultaneous Determination of 32 Pyrrolizidine Alkaloids in Two Traditional Chinese Medicine Preparations by UPLC-MS/MS

Table 5

Mean recoveries and repeatability of the method for PAs in the XYT.

Compound	Average recovery rate at 3 levels of standard addition (%) ± RSD (%) (each n = 9 samples)
	XYT	Extract 1	Extract 2	Atractylodis Macrocephalae Rhizoma	Paeoniae Radix Alba	Bupleuri Radix	Angelicae Sinensis Radix	Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle
Em	100.9 ± 10.8	95.2 ± 12.2	106.9 ± 8.2	83.3 ± 9.7	79.1 ± 13.9	117.1 ± 4.9	87.1 ± 7.8	90.1 ± 8.8
Er	82.2 ± 8.8	88.1 ± 13.9	94.9 ± 12.3	85.9 ± 2.5	74.6 ± 4.3	100.4 ± 9.8	83.0 ± 6.8	93.9 ± 12.4
Eu	95.8 ± 3.1	89.9 ± 2.8	85.8 ± 9.0	91.7 ± 7.3	95.4 ± 7.4	105.5 ± 8.2	97.4 ± 5.7	92.4 ± 7.1
He	97.0 ± 4.2	94.8 ± 6.2	95.7 ± 7.6	95.0 ± 4.9	96.4 ± 4.2	110.1 ± 6.4	94.4 ± 5.9	98.8 ± 9.3
Ic	96.0 ± 8.7	93.6 ± 10.4	89.2 ± 4.9	97.8 ± 4.1	98.7 ± 4.3	112.5 ± 4.1	96.2 ± 6.9	103.6 ± 9.4
Im	96.4 ± 12.1	97.1 ± 11.5	96.6 ± 9.9	99.7 ± 5.3	103.1 ± 11.8	118.5 ± 2.7	97.6 ± 7.4	100.4 ± 10.0
Jb	84.7 ± 11.6	86.8 ± 11.2	99.4 ± 9.5	88.5 ± 5.5	82.2 ± 9.8	102.3 ± 7.7	80.0 ± 9.7	90.6 ± 12.2
Lc	90.4 ± 11.9	73.8 ± 9.0	87.9 ± 8.4	82.9 ± 8.7	91.8 ± 14.0	111.2 ± 10.1	81.5 ± 10.5	96.8 ± 14.7
Ly	96.5 ± 11.5	96.2 ± 10.7	92.6 ± 11.9	95.6 ± 4.9	99.2 ± 5.7	119.2 ± 3.1	98.4 ± 5.4	91.3 ± 9.7
Mc	80.5 ± 12.0	84.9 ± 12.4	85.7 ± 14.3	86.1 ± 7.9	88.3 ± 11.0	100.2 ± 7.9	88.1 ± 14.3	89.1 ± 10.6
Re	91.4 ± 7.0	87.1 ± 12.1	99.2 ± 9.7	85.4 ± 5.4	88.1 ± 6.5	113.5 ± 7.2	104.5 ± 7.9	96.8 ± 8.2
Sk	98.9 ± 14.7	87.2 ± 10.1	97.9 ± 8.9	93.3 ± 7.7	82.2 ± 11.9	114.6 ± 11.5	87.2 ± 12.6	98.2 ± 13.2
Sn	97.5 ± 6.9	90.6 ± 9.7	106.1 ± 11.5	91.3 ± 4.0	90.5 ± 4.8	108.0 ± 9.3	88.8 ± 7.4	100.7 ± 5.9
Sp	94.0 ± 2.8	84.3 ± 8.2	93.8 ± 13.1	83.9 ± 4.1	84.9 ± 8.0	89.8 ± 7.9	81.5 ± 6.7	90.0 ± 12.3
Sv	92.0 ± 6.0	89.1 ± 12.2	97.9 ± 9.5	89.9 ± 3.9	90.0 ± 6.4	105.1 ± 5.3	86.4 ± 7.2	101.7 ± 9.2
Td	88.9 ± 7.7	85.8 ± 9.6	84.1 ± 8.3	90.1 ± 4.0	91.4 ± 6.9	96.3 ± 6.0	95.7 ± 3.6	92.7 ± 4.2
7-Ly	91.3 ± 4.1	105.1 ± 8.3	77.9 ± 12.0	87.2 ± 4.8	89.3 ± 11.6	78.1 ± 9.7	80.5 ± 8.7	82.1 ± 3.3
7-Im	92.41 ± 6.7	100.5 ± 7.1	80.3 ± 13.5	100.0 ± 3.9	84.6 ± 5.3	86.7 ± 10.8	83.1 ± 8.2	85.9 ± 7.9
EmNO	76.0 ± 12.9	103.6 ± 14.5	91.8 ± 8.6	86.9 ± 9.2	92.9 ± 12.9	82.0 ± 12.1	85.2 ± 9.4	93.0 ± 13.9
ErNO	75.9 ± 6.6	109.4 ± 7.9	84.7 ± 8.3	92.8 ± 5.2	95.8 ± 11.9	80.5 ± 9.7	95.5 ± 10.2	84.8 ± 8.3
EuNO	96.4 ± 7.0	100.4 ± 7.3	91.5 ± 6.9	91.4 ± 7.0	101.6 ± 6.6	97.5 ± 10.4	104.1 ± 8.9	95.2 ± 11.8
HeNO	98.5 ± 3.8	101.0 ± 3.2	91.9 ± 4.3	97.0 ± 3.6	100.1 ± 4.4	97.9 ± 5.5	103.8 ± 5.8	97.5 ± 6.6
IcNO	99.3 ± 3.5	105.5 ± 6.5	89.7 ± 11.4	99.0 ± 2.1	105.2 ± 4.6	92.6 ± 6.8	96.4 ± 6.9	93.7 ± 11.3
ImNO	103.7 ± 13.0	100.4 ± 12.9	91.7 ± 9.2	100.0 ± 6.5	101.7 ± 7.7	91.3 ± 11.6	109.1 ± 12.7	97.9 ± 11.4
JbNO	75.4 ± 7.7	100.7 ± 7.0	74.9 ± 7.3	88.7 ± 8.0	96.3 ± 10.0	78.5 ± 9.4	104.0 ± 8.9	85.5 ± 7.5
LcNO	84.2 ± 15.4	91.8 ± 11.7	90.4 ± 9.8	94.8 ± 7.7	88.8 ± 12.6	83.2 ± 12.9	86.0 ± 13.9	80.2 ± 11.6
LyNO	98.4 ± 9.0	111.8 ± 9.5	86.0 ± 8.8	97.0 ± 4.4	109.2 ± 5.4	100.6 ± 8.5	102.6 ± 9.4	90.6 ± 9.7
McNO	83.6 ± 5.0	101.5 ± 7.8	78.5 ± 9.4	91.3 ± 5.0	103.1 ± 9.5	80.8 ± 7.5	90.4 ± 4.9	85.2 ± 8.6
ReNO	93.8 ± 8.3	100.2 ± 9.4	88.3 ± 6.9	95.5 ± 9.6	102.5 ± 15.2	94.1 ± 9.9	99.7 ± 5.8	95.9 ± 12.2
SnNO	95.7 ± 3.2	107.5 ± 4.8	80.3 ± 9.1	98.6 ± 5.9	106.8 ± 9.3	90.4 ± 12.3	94.8 ± 5.3	97.5 ± 9.6
ReNO	93.8 ± 8.3	100.2 ± 9.4	88.3 ± 6.9	95.5 ± 9.6	102.5 ± 15.2	94.1 ± 9.9	99.7 ± 5.8	95.9 ± 12.2
SnNO	95.7 ± 3.2	107.5 ± 4.8	80.3 ± 9.1	98.6 ± 5.9	106.8 ± 9.3	90.4 ± 12.3	94.8 ± 5.3	97.5 ± 9.6