Simultaneous Determination of 32 Pyrrolizidine Alkaloids in Two Traditional Chinese Medicine Preparations by UPLC-MS/MS

Table 4

Mean recoveries and precision of the method for PAs in various matrices.

Compound	Average recovery rate (%) at intermediate level ± RSD (%)（each n = 6 samples）
	XYT	Extract 1	Extract 2	Atractylodis Macrocephalae Rhizoma	Paeoniae Radix Alba	Bupleuri Radix	Angelicae Sinensis Radix	Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle
Em	103.0 ± 9.9	99.9 ± 6.4	104.0 ± 7.2	89.3 ± 8.7	81.9 ± 5.2	108.2 ± 8.1	90.7 ± 6.2	91.3 ± 5.8
Er	83.1 ± 6.3	87.3 ± 2.0	94.6 ± 9.6	86.3 ± 2.0	81.8 ± 14.1	88.6 ± 6.5	87.9 ± 5.5	83.7 ± 2.4
Eu	95.5 ± 3.0	85.9 ± 7.8	90.3 ± 8.0	95.1 ± 5.4	94.0 ± 4.1	99.0 ± 4.3	98.2 ± 5.5	89.2 ± 3.0
He	93.6 ± 2.5	91.0 ± 6.5	98.6 ± 4.1	95.7 ± 1.6	97.5 ± 3.6	102.0 ± 4.1	98.1 ± 4.0	96.6 ± 1.8
Ic	92.3 ± 2.6	93.4 ± 8.6	92.5 ± 8.8	96.1 ± 4.0	102.7 ± 3.6	106.5 ± 6.2	95.2 ± 2.4	92.9 ± 10.2
Im	90.6 ± 5.8	96.8 ± 11.4	98.4 ± 6.6	101.9 ± 3.3	110.3 ± 6.0	115.3 ± 6.8	102.0 ± 5.3	97.9 ± 7.7
Jb	87.7 ± 8.2	87.0 ± 3.7	95.3 ± 6.9	92.7 ± 6.8	85.9 ± 13.9	95.4 ± 9.8	84.6 ± 3.2	87.1 ± 4.3
Lc	88.5 ± 8.9	78.7 ± 14.2	91.4 ± 8.3	87.5 ± 7.7	90.1 ± 4.5	95.3 ± 13.1	83.5 ± 7.9	81.7 ± 10.8
Ly	98.7 ± 4.6	99.5 ± 10.5	92.5 ± 9.7	96.5 ± 3.2	102.4 ± 6.5	110.2 ± 8.2	101.0 ± 6.5	82.5 ± 4.6
Mc	83.7 ± 11.9	91.9 ± 11.2	84.6 ± 12.0	90.4 ± 6.6	97.0 ± 9.2	95.3 ± 7.2	92.0 ± 12.4	81.9 ± 5.8
Re	97.1 ± 1.3	90.9 ± 8.1	99.7 ± 8.1	90.6 ± 4.0	96.7 ± 7.5	107.3 ± 4.3	103.3 ± 8.9	93.4 ± 2.9
Sk	100.4 ± 12.7	106.4 ± 8.7	90.6 ± 4.1	99.0 ± 6.9	88.5 ± 8.7	104.9 ± 14.9	85.4 ± 6.0	97.8 ± 14.0
Sn	95.9 ± 4.8	90.2 ± 12.1	106.2 ± 10.9	91.4 ± 5.3	93.2 ± 6.7	100.5 ± 2.9	95.4 ± 3.4	96.7 ± 8.4
Sp	92.8 ± 3.0	86.6 ± 5.1	89.9 ± 8.5	85.8 ± 5.3	90.0 ± 8.8	96.7 ± 9.7	86.3 ± 7.9	86.6 ± 7.0
Sv	92.3 ± 4.6	96.4 ± 4.0	96.2 ± 7.2	91.3 ± 1.8	91.6 ± 7.1	103.1 ± 1.9	93.3 ± 2.1	92.3 ± 5.1
Td	90.1 ± 4.9	85.0 ± 12.1	89.7 ± 8.8	91.0 ± 2.7	95.6 ± 6.8	96.8 ± 2.9	97.4 ± 3.6	90.7 ± 7.0
7-Ly	90.3 ± 5.4	81.4 ± 11.9	76.9 ± 5.7	87.9 ± 3.9	88.0 ± 7.0	83.3 ± 8.1	86.0 ± 10.6	82.3 ± 2.4
7-Im	93.5 ± 2.7	86.7 ± 6.9	83.2 ± 9.6	96.9 ± 3.7	85.5 ± 3.5	95.6 ± 13.9	88.1 ± 4.5	83.4 ± 7.1
EmNO	74.6 ± 4.8	88.9 ± 11.8	92.6 ± 7.3	92.1 ± 10.4	95.0 ± 14.2	88.9 ± 12.5	88.8 ± 11.0	95.3 ± 14.9
ErNO	78.5 ± 4.4	106.4 ± 11.4	94.0 ± 8.8	92.7 ± 3.0	102.8 ± 6.1	85.9 ± 8.1	99.5 ± 5.6	90.3 ± 4.4
EuNO	93.5 ± 6.8	96.1 ± 7.6	98.2 ± 14.7	96.6 ± 5.0	104.3 ± 4.5	99.2 ± 7.3	100.0 ± 9.6	94.8 ± 10.0
HeNO	100.1 ± 2.2	100.5 ± 2.9	97.8 ± 4.9	97.8 ± 3.1	101.7 ± 4.3	99.1 ± 1.7	102.5 ± 2.1	98.4 ± 1.8
IcNO	96.0 ± 3.6	106.9 ± 3.7	101.1 ± 10.2	101.7 ± 4.4	105.7 ± 5.3	96.0 ± 4.2	101.0 ± 4.8	98.3 ± 3.1
ImNO	101.7 ± 16.5	99.0 ± 11.5	93.9 ± 11.5	97.4 ± 5.2	103.5 ± 4.4	98.0 ± 5.6	100.1 ± 14.5	98.6 ± 8.7
JbNO	75.6 ± 7.0	107.4 ± 13.3	83.1 ± 11.7	91.7 ± 6.7	103.0 ± 5.6	89.0 ± 6.0	108.1 ± 6.0	94.3 ± 5.6
LcNO	92.4 ± 15.7	98.1 ± 9.2	92.0 ± 6.3	94.2 ± 5.8	93.9 ± 14.6	92.7 ± 13.2	88.0 ± 14.9	78.9 ± 12.9
LyNO	99.0 ± 6.9	111.8 ± 9.9	94.1 ± 12.7	100.1 ± 5.1	109.1 ± 5.4	101.6 ± 3.5	102.1 ± 7.4	97.7 ± 7.1
McNO	85.2 ± 6.5	106.8 ± 8.4	86.5 ± 12.7	94.7 ± 6.2	104.4 ± 9.2	83.9 ± 7.2	96.4 ± 5.0	87.2 ± 11.6
ReNO	96.2 ± 4.4	108.5 ± 7.8	96.4 ± 10.1	103.3 ± 1.8	110.9 ± 4.1	101.5 ± 2.3	105.0 ± 5.4	103.9 ± 3.9
SnNO	94.9 ± 3.9	110.0 ± 7.4	90.1 ± 14.5	104.4 ± 3.6	110.6 ± 5.9	98.7 ± 1.2	96.2 ± 4.8	99.2 ± 6.6
SpNO	76.1 ± 4.0	82.0 ± 8.4	83.9 ± 5.3	83.9 ± 6.9	86.9 ± 10.2	76.6 ± 10.2	80.1 ± 7.7	80.7 ± 8.3
SvNO	92.6 ± 2.4	110.9 ± 7.9	90.3 ± 14.8	101.4 ± 5.8	110.0 ± 5.5	96.2 ± 3.9	92.5 ± 4.4	95.7 ± 6.8