Research Article
Simultaneous Determination of 32 Pyrrolizidine Alkaloids in Two Traditional Chinese Medicine Preparations by UPLC-MS/MS
Table 4
Mean recoveries and precision of the method for PAs in various matrices.
| Compound | Average recovery rate (%) at intermediate level ± RSD (%)(each n = 6 samples) | XYT | Extract 1 | Extract 2 | Atractylodis Macrocephalae Rhizoma | Paeoniae Radix Alba | Bupleuri Radix | Angelicae Sinensis Radix | Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle |
| Em | 103.0 ± 9.9 | 99.9 ± 6.4 | 104.0 ± 7.2 | 89.3 ± 8.7 | 81.9 ± 5.2 | 108.2 ± 8.1 | 90.7 ± 6.2 | 91.3 ± 5.8 | Er | 83.1 ± 6.3 | 87.3 ± 2.0 | 94.6 ± 9.6 | 86.3 ± 2.0 | 81.8 ± 14.1 | 88.6 ± 6.5 | 87.9 ± 5.5 | 83.7 ± 2.4 | Eu | 95.5 ± 3.0 | 85.9 ± 7.8 | 90.3 ± 8.0 | 95.1 ± 5.4 | 94.0 ± 4.1 | 99.0 ± 4.3 | 98.2 ± 5.5 | 89.2 ± 3.0 | He | 93.6 ± 2.5 | 91.0 ± 6.5 | 98.6 ± 4.1 | 95.7 ± 1.6 | 97.5 ± 3.6 | 102.0 ± 4.1 | 98.1 ± 4.0 | 96.6 ± 1.8 | Ic | 92.3 ± 2.6 | 93.4 ± 8.6 | 92.5 ± 8.8 | 96.1 ± 4.0 | 102.7 ± 3.6 | 106.5 ± 6.2 | 95.2 ± 2.4 | 92.9 ± 10.2 | Im | 90.6 ± 5.8 | 96.8 ± 11.4 | 98.4 ± 6.6 | 101.9 ± 3.3 | 110.3 ± 6.0 | 115.3 ± 6.8 | 102.0 ± 5.3 | 97.9 ± 7.7 | Jb | 87.7 ± 8.2 | 87.0 ± 3.7 | 95.3 ± 6.9 | 92.7 ± 6.8 | 85.9 ± 13.9 | 95.4 ± 9.8 | 84.6 ± 3.2 | 87.1 ± 4.3 | Lc | 88.5 ± 8.9 | 78.7 ± 14.2 | 91.4 ± 8.3 | 87.5 ± 7.7 | 90.1 ± 4.5 | 95.3 ± 13.1 | 83.5 ± 7.9 | 81.7 ± 10.8 | Ly | 98.7 ± 4.6 | 99.5 ± 10.5 | 92.5 ± 9.7 | 96.5 ± 3.2 | 102.4 ± 6.5 | 110.2 ± 8.2 | 101.0 ± 6.5 | 82.5 ± 4.6 | Mc | 83.7 ± 11.9 | 91.9 ± 11.2 | 84.6 ± 12.0 | 90.4 ± 6.6 | 97.0 ± 9.2 | 95.3 ± 7.2 | 92.0 ± 12.4 | 81.9 ± 5.8 | Re | 97.1 ± 1.3 | 90.9 ± 8.1 | 99.7 ± 8.1 | 90.6 ± 4.0 | 96.7 ± 7.5 | 107.3 ± 4.3 | 103.3 ± 8.9 | 93.4 ± 2.9 | Sk | 100.4 ± 12.7 | 106.4 ± 8.7 | 90.6 ± 4.1 | 99.0 ± 6.9 | 88.5 ± 8.7 | 104.9 ± 14.9 | 85.4 ± 6.0 | 97.8 ± 14.0 | Sn | 95.9 ± 4.8 | 90.2 ± 12.1 | 106.2 ± 10.9 | 91.4 ± 5.3 | 93.2 ± 6.7 | 100.5 ± 2.9 | 95.4 ± 3.4 | 96.7 ± 8.4 | Sp | 92.8 ± 3.0 | 86.6 ± 5.1 | 89.9 ± 8.5 | 85.8 ± 5.3 | 90.0 ± 8.8 | 96.7 ± 9.7 | 86.3 ± 7.9 | 86.6 ± 7.0 | Sv | 92.3 ± 4.6 | 96.4 ± 4.0 | 96.2 ± 7.2 | 91.3 ± 1.8 | 91.6 ± 7.1 | 103.1 ± 1.9 | 93.3 ± 2.1 | 92.3 ± 5.1 | Td | 90.1 ± 4.9 | 85.0 ± 12.1 | 89.7 ± 8.8 | 91.0 ± 2.7 | 95.6 ± 6.8 | 96.8 ± 2.9 | 97.4 ± 3.6 | 90.7 ± 7.0 | 7-Ly | 90.3 ± 5.4 | 81.4 ± 11.9 | 76.9 ± 5.7 | 87.9 ± 3.9 | 88.0 ± 7.0 | 83.3 ± 8.1 | 86.0 ± 10.6 | 82.3 ± 2.4 | 7-Im | 93.5 ± 2.7 | 86.7 ± 6.9 | 83.2 ± 9.6 | 96.9 ± 3.7 | 85.5 ± 3.5 | 95.6 ± 13.9 | 88.1 ± 4.5 | 83.4 ± 7.1 | EmNO | 74.6 ± 4.8 | 88.9 ± 11.8 | 92.6 ± 7.3 | 92.1 ± 10.4 | 95.0 ± 14.2 | 88.9 ± 12.5 | 88.8 ± 11.0 | 95.3 ± 14.9 | ErNO | 78.5 ± 4.4 | 106.4 ± 11.4 | 94.0 ± 8.8 | 92.7 ± 3.0 | 102.8 ± 6.1 | 85.9 ± 8.1 | 99.5 ± 5.6 | 90.3 ± 4.4 | EuNO | 93.5 ± 6.8 | 96.1 ± 7.6 | 98.2 ± 14.7 | 96.6 ± 5.0 | 104.3 ± 4.5 | 99.2 ± 7.3 | 100.0 ± 9.6 | 94.8 ± 10.0 | HeNO | 100.1 ± 2.2 | 100.5 ± 2.9 | 97.8 ± 4.9 | 97.8 ± 3.1 | 101.7 ± 4.3 | 99.1 ± 1.7 | 102.5 ± 2.1 | 98.4 ± 1.8 | IcNO | 96.0 ± 3.6 | 106.9 ± 3.7 | 101.1 ± 10.2 | 101.7 ± 4.4 | 105.7 ± 5.3 | 96.0 ± 4.2 | 101.0 ± 4.8 | 98.3 ± 3.1 | ImNO | 101.7 ± 16.5 | 99.0 ± 11.5 | 93.9 ± 11.5 | 97.4 ± 5.2 | 103.5 ± 4.4 | 98.0 ± 5.6 | 100.1 ± 14.5 | 98.6 ± 8.7 | JbNO | 75.6 ± 7.0 | 107.4 ± 13.3 | 83.1 ± 11.7 | 91.7 ± 6.7 | 103.0 ± 5.6 | 89.0 ± 6.0 | 108.1 ± 6.0 | 94.3 ± 5.6 | LcNO | 92.4 ± 15.7 | 98.1 ± 9.2 | 92.0 ± 6.3 | 94.2 ± 5.8 | 93.9 ± 14.6 | 92.7 ± 13.2 | 88.0 ± 14.9 | 78.9 ± 12.9 | LyNO | 99.0 ± 6.9 | 111.8 ± 9.9 | 94.1 ± 12.7 | 100.1 ± 5.1 | 109.1 ± 5.4 | 101.6 ± 3.5 | 102.1 ± 7.4 | 97.7 ± 7.1 | McNO | 85.2 ± 6.5 | 106.8 ± 8.4 | 86.5 ± 12.7 | 94.7 ± 6.2 | 104.4 ± 9.2 | 83.9 ± 7.2 | 96.4 ± 5.0 | 87.2 ± 11.6 | ReNO | 96.2 ± 4.4 | 108.5 ± 7.8 | 96.4 ± 10.1 | 103.3 ± 1.8 | 110.9 ± 4.1 | 101.5 ± 2.3 | 105.0 ± 5.4 | 103.9 ± 3.9 | SnNO | 94.9 ± 3.9 | 110.0 ± 7.4 | 90.1 ± 14.5 | 104.4 ± 3.6 | 110.6 ± 5.9 | 98.7 ± 1.2 | 96.2 ± 4.8 | 99.2 ± 6.6 | SpNO | 76.1 ± 4.0 | 82.0 ± 8.4 | 83.9 ± 5.3 | 83.9 ± 6.9 | 86.9 ± 10.2 | 76.6 ± 10.2 | 80.1 ± 7.7 | 80.7 ± 8.3 | SvNO | 92.6 ± 2.4 | 110.9 ± 7.9 | 90.3 ± 14.8 | 101.4 ± 5.8 | 110.0 ± 5.5 | 96.2 ± 3.9 | 92.5 ± 4.4 | 95.7 ± 6.8 |
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