Research Article

A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres

Table 2

Comparison results of serum samples using the proposed and ELISA method.

Serum sampleProposed methoda (ng·mL−1)ELISAa (ng·mL−1)Relative deviation (%)

11 0.116 ± 0.0120.113 ± 0.0082.65
22 0.308 ± 0.0180.316 ± 0.014−2.53
30.653 ± 0.0110.689 ± 0.015−5.22
41.152 ± 0.0091.138 ± 0.0211.23
55.210 ± 0.0255.002 ± 0.0344.16
615.262 ± 0.13814.806 ± 0.1123.08
724.139 ± 0.16525.418 ± 0.104−5.03
843.615 ± 0.21342.221 ± 0.2263.30

aMean value ± SD of five measurements serum sample.