Lipid Profiling of Pacific Abalone (Haliotis discus hannai) at Different Developmental Stages Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Table 1

Comparison between the current study and previously published lipidomics studies of abalone (Haliotis discus hannai) tissue.

References	Extraction	Method	Software/libraries	Key findings (level identification)
Current study	(i) Neutral extraction CHCl3/MeOH	UHPLC-Q-Orbitrap-MS/MS	Lipidsearch	(i) 132 lipid species were identified
				(ii) The lipid composition of tissue at three different developmental stages included 15 lipid classes (TG, PC, etc)
	(ii) Acidic extraction CHCl3/MeOH/37% (1N) HCl
Zhang et al., 2018 [33]	Folch method	UPLC-ESI-Q-TOF-MS	MS-DIAL and LIPID MAPS	(i) Thirty-four lipid species were identified (10 lipid classes)
Lou et al., 2013 [13]	Folch method	GC-MS	Wiley and NIST libraries	(i) The lipid composition of muscle and viscera tissues included total cholesterol, triacylglycerol, free fatty acids, and phospholipids
				(ii) Thirty-two fatty acids were identified
Zhou et al., 2012 [69]	(i) Soxhlet extraction	(i) TLC-FID (lipids)	NIST02 libraries	(i) The lipid composition of gonad tissue included TAG, phospholipids, cholesterol, and FAA
	(ii) SC-CO2 extraction	GC-MS (unsaponifiable/fatty acid methyl esters)		(ii) Composition of unsaponifiable lipids (4 sterol species) was examined
	(iii) Enzyme-assisted organic solvent method			(iii) Thirteen saponifiable fatty acids were identified
				(iv) Percent composition depends on the extraction method