Research Article
Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach
Table 3
Stability of diosgenin for the determination of the three concentrations in rat plasma (n = 6).
| | Concentration (ng/ml) | RSD/% | Indicated | Measured |
| Short-term stability at 25°C for 8 h | 5 | 5.41 ± 0.33 | 6.09 | 50 | 50.23 ± 2.56 | 5.10 | 1000 | 1000.88 ± 44.34 | 4.43 |
| Long-term stability at −20°C for 30 days | 5 | 5.99 ± 0.28 | 4.67 | 50 | 50.02 ± 3.72 | 7.43 | 1000 | 1001.65 ± 42.42 | 4.23 |
| Freeze-thaw stability, three cycles | 5 | 5.98 ± 0.35 | 5.85 | 50 | 51.69 ± 4.36 | 8.43 | 1000 | 1002.9 ± 54.44 | 5.42 |
| Autosampler stability at 4°C for 24 h | 5 | 5.32 ± 0.37 | 6.95 | 50 | 50.59 ± 3.69 | 5.42 | 1000 | 1001.01 ± 62.63 | 5.42 |
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