Measurement of Pharmacokinetics and Tissue Distribution of Four Compounds from Nauclea officinalis in Rat Plasma and Tissues through HPLC-MS/MS

Table 4

Summary of extraction recovery and matrix effect of four analytes in plasma and tissue samples (n = 6).

Compounds	Matrix	Concentration (ng/mL)	Matrix effect (%)	RSD (%)	Extraction recovery (%)	RSD (%)
Chlorogenic acid	Plasma	2	97.26 ± 5.35	5.50	80.45 ± 4.36	5.42
		4	109.75 ± 4.40	4.01	80.04 ± 3.08	3.85
		100	82.80 ± 6.51	7.86	69.49 ± 4.05	5.84
		2400	91.66 ± 1.53	1.67	72.26 ± 1.16	1.61
	Heart	2	116.54 ± 3.03	2.60	80.53 ± 9.62	11.95
		4	98.55 ± 2.71	2.75	76.82 ± 4.77	6.21
		100	97.78 ± 1.76	1.80	62.02 ± 2.24	3.61
		2400	93.25 ± 0.76	0.81	63.32 ± 0.58	0.91
	Liver	2	83.69 ± 4.24	5.06	97.06 ± 3.75	3.86
		4	79.96 ± 4.79	5.94	79.07 ± 2.90	3.66
		100	63.82 ± 2.51	3.94	66.44 ± 0.24	0.37
		2400	80.01 ± 0.90	1.12	69.83 ± 1.55	2.22
	Spleen	2	99.89 ± 2.61	2.61	80.09 ± 1.22	1.52
		4	100.56 ± 1.99	1.98	86.82 ± 2.88	3.32
		100	92.05 ± 1.01	1.10	67.29 ± 1.81	2.69
		2400	91.66 ± 0.81	0.88	73.12 ± 1.61	2.20
	Lung	2	104.73 ± 1.78	1.70	81.80 ± 2.52	3.08
		4	99.34 ± 2.07	2.09	88.65 ± 1.55	1.75
		100	98.65 ± 1.24	1.25	80.24 ± 2.11	2.63
		2400	96.68 ± 1.81	1.88	76.89 ± 1.69	2.20
	Kidney	2	90.49 ± 5.66	6.26	78.34 ± 4.02	5.13
		4	98.52 ± 4.21	4.27	86.92 ± 3.00	3.46
		100	95.03 ± 5.94	6.25	70.73 ± 4.90	6.92
		2400	89.55 ± 2.38	3.16	72.70 ± 1.50	2.07
	Small intestine	2	56.96 ± 6.16	10.81	56.20 ± 3.36	5.98
		4	63.67 ± 2.46	4.14	51.85 ± 1.94	3.73
		100	53.67 ± 1.06	1.97	61.26 ± 1.87	3.05
		2400	50.17 ± 1.72	3.42	62.66 ± 1.06	1.70
	Stomach	2	114.40 ± 5.01	4.46	85.33 ± 5.20	6.10
		4	116.62 ± 8.80	7.55	91.66 ± 6.11	6.67
		100	102.87 ± 3.24	3.15	66.48 ± 3.94	5.93
		2400	98.38 ± 1.63	1.65	69.41 ± 1.56	2.25
	Brain	2	97.89 ± 6.42	6.56	82.33 ± 4.65	5.65
		4	105.09 ± 2.99	2.84	75.80 ± 3.59	4.74
		100	90.02 ± 5.46	6.06	71.37 ± 1.17	1.63
		2400	90.56 ± 2.16	2.39	86.06 ± 1.68	1.95
Naucleactonin C	Plasma	5	104.57 ± 6.94	6.64	100.30 ± 11.77	11.74
		8	102.54 ± 2.55	2.48	106.68 ± 6.31	5.91
		140	104.07 ± 2.88	2.77	96.41 ± 1.66	1.72
		2400	102.64 ± 2.09	2.04	88.27 ± 1.05	1.19
	Heart	5	98.86 ± 2.67	2.70	97.71 ± 1.95	2.00
		8	99.69 ± 3.96	3.97	96.33 ± 7.19	7.46
		140	106.24 ± 4.87	4.58	85.37 ± 2.90	3.40
		2400	99.81 ± 1.48	1.48	86.76 ± 1.61	1.86
	Liver	5	101.81 ± 10.88	10.69	92.06 ± 9.87	10.72
		8	111.16 ± 3.12	2.81	85.03 ± 4.91	5.77
		140	97.92 ± 5.09	5.20	84.88 ± 4.52	5.33
		2400	97.02 ± 1.11	1.14	80.54 ± 0.83	1.03
	Spleen	5	90.83 ± 3.65	4.02	100.21 ± 9.21	9.19
		8	93.78 ± 6.07	6.47	99.90 ± 5.49	5.50
		140	97.28 ± 2.71	2.79	84.00 ± 1.54	1.83
		2400	100.67 ± 0.79	0.78	84.60 ± 1.40	1.65
	Lung	5	98.17 ± 7.63	7.77	105.63 ± 3.87	3.66
		8	115.99 ± 7.97	5.01	99.21 ± 12.43	12.53
		140	98.73 ± 1.39	1.41	95.48 ± 2.88	3.01
		2400	99.21 ± 0.91	0.92	84.40 ± 1.82	2.15
	Kidney	5	99.90 ± 5.10	5.10	94.08 ± 8.11	8.62
		8	100.35 ± 5.14	5.12	105.08 ± 5.22	4.97
		140	101.18 ± 0.70	0.69	86.02 ± 4.39	5.10
		2400	102.49 ± 1.87	1.83	86.64 ± 2.26	2.60
	Small intestine	5	98.00 ± 5.51	5.62	98.55 ± 9.61	9.75
		8	99.37 ± 8.48	8.90	82.00 ± 5.03	6.14
		140	95.42 ± 5.52	5.79	91.73 ± 1.67	1.82
		2400	86.75 ± 2.24	2.58	86.61 ± 3.15	3.63
	Stomach	5	101.53 ± 3.55	3.50	97.93 ± 4.19	4.28
		8	101.21 ± 3.87	3.82	99.25 ± 3.17	3.20
		140	99.62 ± 1.19	1.20	82.46 ± 1.88	2.28
		2400	101.37 ± 1.35	1.33	75.99 ± 2.77	3.64
	Brain	5	97.50 ± 6.50	6.67	88.08 ± 3.12	3.54
		8	105.47 ± 5.14	4.87	88.38 ± 7.32	8.29
		140	102.02 ± 0.85	0.83	97.63 ± 4.35	4.46
		2400	101.24 ± 2.44	2.41	99.47 ± 1.42	1.43
Khaephuoside A	Plasma	5	64.06 ± 5.66	8.84	88.84 ± 5.10	5.75
		8	65.86 ± 5.65	8.57	92.35 ± 8.73	9.45
		120	55.55 ± 4.05	7.29	85.88 ± 2.06	2.40
		1800	58.01 ± 1.93	3.33	85.24 ± 3.30	3.87
	Heart	5	70.92 ± 8.09	11.41	96.07 ± 5.37	5.59
		8	77.13 ± 3.56	4.61	82.29 ± 3.95	4.80
		120	54.79 ± 1.14	2.08	92.18 ± 1.64	1.78
		1800	59.93 ± 4.64	7.75	86.98 ± 4.87	5.59
	Liver	5	73.90 ± 6.84	9.26	105.35 ± 11.02	10.46
		8	53.20 ± 1.59	2.98	103.34 ± 3.75	3.63
		120	58.17 ± 1.12	1.93	77.46 ± 2.17	2.80
		1800	61.29 ± 2.80	4.58	84.49 ± 1.70	2.01
	Spleen	5	68.33 ± 1.41	2.07	90.99 ± 7.89	8.67
		8	60.36 ± 2.58	4.28	96.38 ± 2.77	2.88
		120	58.12 ± 0.42	0.73	90.31 ± 2.80	3.10
		1800	61.44 ± 1.13	1.84	86.75 ± 0.98	1.13
	Lung	5	84.39 ± 4.20	4.97	77.71 ± 3.73	4.81
		8	72.06 ± 6.89	9.56	87.06 ± 7.30	8.38
		120	59.00 ± 2.55	4.33	86.34 ± 3.48	4.03
		1800	61.35 ± 4.41	7.19	79.39 ± 6.48	8.17
	Kidney	5	57.46 ± 1.80	3.13	97.31 ± 4.38	4.50
		8	51.41 ± 2.91	5.66	99.78 ± 3.41	3.42
		120	45.79 ± 1.30	2.84	99.65 ± 1.23	1.23
		1800	47.23 ± 1.38	2.93	101.56 ± 2.17	2.14
	Small intestine	5	56.14 ± 1.47	2.61	102.93 ± 10.44	10.15
		8	67.00 ± 5.43	8.10	94.63 ± 6.27	6.63
		120	62.69 ± 2.06	3.29	100.39 ± 5.00	4.98
		1800	71.51 ± 2.08	2.91	99.78 ± 1.06	1.06
	Stomach	5	56.84 ± 3.21	5.64	95.49 ± 6.60	6.91
		8	63.57 ± 5.59	8.80	83.81 ± 6.89	8.22
		120	54.53 ± 2.44	4.48	77.49 ± 4.08	5.27
		1800	55.57 ± 1.07	1.92	79.96 ± 0.87	1.09
	Brain	5	76.07 ± 1.69	2.22	93.48 ± 8.70	9.30
		8	76.50 ± 6.23	8.15	102.39 ± 6.80	6.64
		120	87.44 ± 3.23	3.70	90.50 ± 0.99	1.09
		1800	79.81 ± 2.15	2.70	100.48 ± 1.42	1.41
3,4-Dimethoxyphenyl-1-O-β-apiofuroseyl(1 ⟶ 2)-β-D-glucopyranoside	Plasma	25	64.80 ± 4.95	7.64	88.62 ± 8.56	9.66
		50	65.25 ± 0.81	1.24	89.23 ± 4.24	4.76
		350	60.35 ± 3.67	6.08	85.65 ± 1.17	1.37
		2400	55.98 ± 2.34	4.18	84.82 ± 3.16	3.72
	Heart	25	79.81 ± 2.39	2.99	80.56 ± 4.05	5.03
		50	73.97 ± 1.84	2.49	89.91 ± 2.89	3.21
		350	69.51 ± 4.04	5.81	85.34 ± 4.20	4.92
		2400	64.96 ± 2.28	3.51	85.00 ± 3.15	3.71
	Liver	25	55.02 ± 4.02	7.31	88.70 ± 8.38	9.45
		50	53.00 ± 1.63	3.08	94.52 ± 4.76	5.04
		350	52.69 ± 2.12	4.03	83.51 ± 2.63	3.15
		2400	51.60 ± 1.44	2.80	87.94 ± 2.88	3.27
	Spleen	25	81.22 ± 3.27	4.02	98.19 ± 2.87	2.92
		50	87.52 ± 4.51	5.16	92.02 ± 3.15	3.42
		350	81.89 ± 1.03	1.26	89.66 ± 2.42	2.70
		2400	80.06 ± 3.07	3.83	80.48 ± 2.15	2.68
	Lung	25	91.11 ± 4.01	4.40	68.36 ± 4.42	6.47
		50	92.34 ± 3.46	3.75	77.05 ± 2.14	2.78
		350	77.28 ± 4.36	5.65	83.53 ± 3.42	4.09
		2400	76.69 ± 2.73	3.56	75.92 ± 0.73	0.97
	Kidney	25	63.39 ± 1.54	2.43	86.96 ± 4.51	5.19
		50	64.43 ± 1.53	2.38	99.12 ± 2.65	2.67
		350	53.76 ± 3.07	5.72	101.00 ± 1.81	1.79
		2400	60.75 ± 1.53	2.51	103.75 ± 3.17	3.05
	Small intestine	25	64.61 ± 4.23	6.55	98.39 ± 11.15	11.33
		50	68.08 ± 3.68	5.40	90.4 ± 3.02	3.34
		350	70.33 ± 6.13	8.71	101.86 ± 5.70	5.60
		2400	64.51 ± 2.35	3.64	99.20 ± 1.01	1.02
	Stomach	25	68.84 ± 1.18	1.72	92.61 ± 3.13	3.38
		50	62.45 ± 2.20	3.52	88.53 ± 3.44	3.89
		350	65.27 ± 1.67	2.56	77.56 ± 1.83	2.36
		2400	62.11 ± 0.87	1.40	83.08 ± 2.16	2.60
	Brain	25	71.85 ± 1.85	2.57	90.54 ± 1.48	1.64
		50	78.40 ± 2.29	2.92	86.20 ± 4.87	5.65
		350	85.96 ± 2.91	3.39	93.34 ± 1.88	2.02
		2400	75.94 ± 1.52	2.00	101.87 ± 1.82	1.78