Research Article

A Human Oral Fluid Assay for D- and L- Isomer Detection of Amphetamine and Methamphetamine Using Liquid-Liquid Extraction

Table 8

Stability testing. QC samples were tested for stability after 3 freeze-thaw cycles. They were also tested overnight at the indicated temperatures. A 3- and 7- day postextraction study were also performed at 2–8°C.

Drug/MetaboliteF/T 3 cyclesOvernight stability (%)Postpreparation stability
QC% DiffRT4°C−20°CInit % diff nom% Diff init day 3% Diff init day 7

AmphetamineQC 750.692.484.913.638.95−1.330.61
QC 8006.926.218.190.45−0.690.16−1.78

MethamphetamineQC 75−2.381.39-0.230.72−1.252.616.13
QC 18001.122.621.954.85−2.22−4.26−1.39

Indicates high QC of 800.