A Human Oral Fluid Assay for D- and L- Isomer Detection of Amphetamine and Methamphetamine Using Liquid-Liquid Extraction

Table 6

Intraassay precision and accuracy: precision and accuracy over 3 days with replicates of 6 for each day.

Drug/Metabolite	LLOQ				LQC				MQC				HQC
	%CV		%E		%CV		%E		%CV		%E		%CV		%E
	MIN	MAX	MIN	MAX	MIN	MAX	MIN	MAX	MIN	MAX	MIN	MAX	MIN	MAX	MIN	MAX
Amphetamine	2.28	10.19	−2.20	13.20	2.72	5.62	9.07	12.43	2.06	5.57	−5.07	−0.91	3.46	8.89	−5.00	6.59
Methamphetamine	4.84	9.89	−2.60	7.53	1.71	4.41	−0.02	1.66	1.71	2.92	−6.31	2.48	2.10	4.41	−2.22	6.50
D-amphetamine	1.38	3.51	8.44	15.79	1.14	1.40	4.38	15.69	0.69	1.73	−2.89	-0.02	1.46	2.99	0.75	7.90
L-amphetamine	2.60	4.47	6.63	13.33	1.11	1.79	9.38	15.71	1.14	1.57	−3.13	0.31	1.46	2.33	−5.16	0.02
D-methamphetamine	1.28	4.42	−1.56	5.73	1.67	2.66	−3.19	4.96	1.75	2.21	−4.28	4.24	1.67	2.74	7.59	17.89
L-methamphetamine	1.66	5.19	−2.83	7.65	2.59	3.56	−3.14	6.69	0.55	0.99	−4.74	7.49	2.17	5.87	8.68	17.91

Indicates combined D- and L-.