Determination of Tropifexor in Beagle Dog Plasma by UPLC-MS/MS and Its Application in Pharmacokinetics

Table 4

Stability of tropifexor in beagle plasma subjected to various conditions (n = 6).

Added (ng/mL)	Room temperature, 4 h		Autosampler 24°C, 24 h		Three freeze–thaw		−80°C, 60 days
	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)
2.5	3.84	0.33	4.44	1.13	5.36	−5.80	4.49	−8.20
50	3.59	−0.97	3.45	0.64	4.87	1.78	2.45	−3.19
150	1.91	0.24	1.94	−0.03	2.57	−1.09	1.07	−2.66