Method Validation and Characterization of Red Pigment in Beta vulgaris Peels and Pomaces by HPLC-UV and UHPLC-MS/MS

Table 6

Betalains identified in Beta vulgaris L. peels and pomaces extract by LC-MS/MS.

Compound #	Compound name	tR [min]	Full-scan MS of [m/z]	MS/MS of [m/z]	Ions	Relative peak area (%) of betalains	Content estimates (mg/g) of dry weight
1.	2-O-Glucosyl-betanin	6.97	389.1	345.1	[M + H]^+	4.02 ± 0.10	0.40 ± 0.10
2.	Betanin (betanidin 5-glucoside)	7.71	551.1	389.1	[M + H]^+	43.12 ± 2.15	4.31 ± 2.15
3.	2-O-Glucosyl-isobetanin	7.98	389.1	345.1	[M + H]^+	0.52 ± 0.08	0.05 ± 0.08
4.	Isobetanin (isobetanidin 5-glucoside)	8.81	551.1	389.1	[M + H]^+	18.45 ± 2.20	1.85 ± 2.20
5	Betanidin	9.46	387.1	321.1, 267.2	[M + H]^+	7.05 ± 0.03	0.71 ± 0.03
6.	Ferulic acid hexoside (4-glucoside)	9.68	355.1	315.1	[M − H]^−	1.03 ± 0.02	0.10 ± 0.02
7.	17-Decarboxy-neobetanin	10.93	431.1	387.1, 267.2	[M + H]^+	0.49 ± 0.07	0.05 ± 0.07
8.	Isobetanidin (17-decarboxy-betanidin)	11.67	345.1	267.1	[M + H]^+	3.60 ± 1.26	0.36 ± 1.26
9.	Neobetanin	12.13	549.1	387.1	[M + H]^+	3.24 ± 0.35	0.32 ± 0.35
10.	17-Decarboxy-betanin	13.72	507.2	343.1, 297.1	[M + H]^+	0.35 ± 0.03	0.04 ± 0.03
11.	2,17-Bidecarboxy-betanin (isobetanin)	14.18	475.1	267.1	[M − H]^−	1.59 ± 0.42	0.16 ± 0.42
12.	2,17-Bidecarboxy -neobetanin	14.78	461.2	299.1	[M + H]^+	7.07 ± 0.02	0.71 ± 0.02
13.	Isovitexin (apigenin 6-C- glucoside)	14.86	433.2	343.1, 267.2	[M + H]^+	2.54 ± 0.19	0.25 ± 0.19

Results are the mean values from five replicates ± S.D.