Method Validation and Characterization of Red Pigment in Beta vulgaris Peels and Pomaces by HPLC-UV and UHPLC-MS/MS

Table 1

Component of mobile phase used in TLC analysis of B. vulgaris L. peels and pomaces.

Component of mobile phase	Ratio (v/v)	Rf
Hexane	1	0.02
Methanol	1	0.14
Hexane : methanol	8 : 2	0.17
Hexane : methanol	3 : 7	0.21
Acetone : ethyl acetate	6 : 4	0.13
Acetone : ethyl acetate	7 : 3	0.16
Acetone : ethyl acetate	3 : 7	0.20
Acetone : hexane	7 : 3	0.19
Acetic acid (glacial) : methanol : water (distilled)	0.7 : 9 : 0.3	0.54
Acetic acid (glacial) : methanol : water (distilled)	1 : 6 : 3	0.41
Acetic acid (glacial) : ethanol : isopropyl : water (distilled)	1 : 2 : 6 : 1	0.32
Acetic acid (glacial) : ethanol : isopropyl : water (distilled)	1 : 4 : 3 : 2	0.29
Acetic acid (glacial) : acetonitrile : methanol : water (distilled)	0.5 : 2 : 7 : 0.5	0.23