Systematic Study on a Quantitative Analysis of Multicomponents by Single Marker (QAMS) Method for Simultaneous Determination of Eight Constituents in Pneumonia Mixture by UPLC-MS/MS
Table 3
Contents of the eight components in the pneumonia mixture determined by ESM, AVG, and LRG methods.
Sample
Baicalein
(R, S)-goitrin
Amygdalin
Chlorogenic acid
ESM (mg/mL)
ESM (mg/mL)
AVG (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
ESM (mg/mL)
AVG## (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
ESM (mg/mL)
AVG (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
1
0.47
0.0045
0.0043
3.81
0.0045
0.27
7.41
7.05
4.89
7.46
0.69
2.02
1.97
2.68
2.02
0.04
2
0.44
0.0040
0.0042
4.59
0.0044
9.02
7.46
7.10
4.90
7.51
0.68
2.07
2.02
2.67
2.08
0.06
3
0.45
0.0040
0.0041
3.41
0.0043
7.79
7.45
7.09
4.90
7.50
0.68
2.10
2.04
2.67
2.10
0.06
4
0.42
0.0040
0.0042
4.37
0.0044
8.80
7.20
6.85
4.86
7.25
0.72
1.99
1.94
2.66
2.00
0.06
5
0.45
0.0045
0.0044
1.65
0.0046
2.52
7.31
6.96
4.89
7.37
0.69
2.09
2.03
2.67
2.09
0.05
6
0.43
0.0040
0.0040
0.36
0.0042
4.61
7.17
6.82
4.86
7.22
0.72
1.98
1.93
2.66
1.99
0.06
Sample
Pseudoephedrine hydrochloride
Ephedrine hydrochloride
Ammonium glycyrrhizinate
Baicalin
ESM (mg/mL)
AVG## (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
ESM (mg/mL)
AVG# (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
ESM (mg/mL)
AVG (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
ESM (mg/mL)
AVG## (mg/mL)
SMD (%)
LRG (mg/mL)
SMD (%)
1
0.39
0.37
4.68
0.39
0.17
0.18
0.17
4.66
0.18
0.20
0.49
0.52
4.53
0.50
0.85
11.55
10.85
6.02
11.68
1.13
2
0.39
0.37
4.78
0.39
0.06
0.17
0.17
4.53
0.18
0.33
0.42
0.44
4.69
0.42
1.00
11.59
10.89
6.02
11.72
1.13
3
0.40
0.38
4.80
0.40
0.04
0.18
0.17
4.65
0.18
0.21
0.42
0.44
4.72
0.42
1.03
11.64
10.94
6.03
11.77
1.12
4
0.38
0.36
4.75
0.38
0.10
0.17
0.16
4.68
0.17
0.18
0.42
0.44
4.76
0.43
1.07
11.23
10.56
5.99
11.36
1.16
5
0.39
0.37
4.76
0.39
0.08
0.18
0.17
4.61
0.18
0.25
0.39
0.41
4.79
0.40
1.10
11.59
10.89
6.02
11.72
1.13
6
0.38
0.36
4.73
0.38
0.12
0.17
0.16
4.50
0.17
0.37
0.40
0.42
4.78
0.41
1.09
11.40
10.71
6.01
11.53
1.14
#P < 0.05 and ##P < 0.01, AVG group vs. ESM group.
