Development of a UPLC-MS/MS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats

Table 1

Precision, accuracy, matrix effect, and recovery for analysis of VS-5584 in rat plasma (n = 6).

Spiked concentration (ng/mL)	Intraday precision CV (%)	Interday precision CV (%)	Accuracy (RE%)	Matrix effect (%, mean ± SD)	Recovery (%, mean ± SD)
1.0	9.2	8.0	−13.6	93.3 ± 6.9	92.4 ± 5.5
2.0	5.9	7.0	−9.5	105.3 ± 6.2	92.0 ± 4.4
200	4.8	7.0	8.8	94.1 ± 1.8	89.4 ± 3.9
800	5.5	5.3	8.2	98.8 ± 1.9	90.8 ± 3.9
IS	—	—	—	90.3 ± 9.8	89.3 ± 3.8