The Importance of Derivatizing Reagent in Chromatography Applications for Biogenic Amine Detection in Food and Beverages

Table 1

The different between solid-phase extraction (SPE) and matrix solid-phase dispersion (MPSD) [14, 15].

No.	SPE technique	MPSD technique
1	A method used to purify analytes from a sample by sorption onto a disposable solid-phase cartridge, followed by elution of the analyte with a solvent before analysis using liquid chromatography; this method can increase the selectivity of LC	A method that involves simultaneous disruption and extraction of various solid and semisolid samples owing to the direct mechanical blending of the sample
2	Sample should be prepared step-by-step, and some of the sample components should be dispersed during the process in order to make sure the analyte is suitable for SPE column	This technique makes sure all the sample components are extracted, and none of them is discarded
3	Only the specific analyte will be detected	As none of the components are discarded, physical and chemical interactions inside the components occur
4	Sample is absorbed onto the top of the column packing material	This technique ensures the sample is not absorbed throughout the column