Research Article
A Simple and Rapid LC-MS/MS Method for Quantification of Total Daidzein, Genistein, and Equol in Human Urine
Table 2
Comparison of the efficiency of conjugate hydrolysis between method 1 and method 2.
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Method 1 is the original method and does not result in complete hydrolysis of isoflavone and equol sulfates. Method 2 is the new method reported here which efficiently hydrolyses all the isoflavone and equol phase-2 conjugates including the sulfates. + denotes presence of compound after hydrolysis (i.e., not fully hydrolysed) and − denotes fully hydrolysed (i.e., no detectable conjugates observed after hydrolysis). |