Determination of Anlotinib, a Tyrosine Kinase Inhibitor, in Rat Plasma by UHPLC-MS/MS and Its Application to a Pharmacokinetic Study

Table 4

Stability of anlotinib in rat plasma under various storage conditions (n = 3).

		Short-term stability	Long-term stability	Freeze/thaw stability	Autosampler stability
0.25 ng/ml	Mean	0.26	0.25	0.26	0.24
	RSD	3.9	2.18	4.1	4.42
	RE	4.65	−1.55	2.03	−2.27
5 ng/ml	Mean	5.21	5.19	4.94	5.14
	RSD	3.99	3.32	3.47	4.32
	RE	4.27	3.75	−1.13	2.75
40 ng/ml	Mean	41.64	41.19	40.55	41.14
	RSD	3.26	3.97	3.91	4.81
	RE	4.1	2.97	1.39	2.85